In nature, there are many tiny organisms that cannot be directly seen by the naked eye and require magnification with a microscope to observe them. These tiny organisms are collectively called microorganisms. The filtration of microorganisms requires the use of filter membranes with very small pore sizes.
Aperture size is usually determined by the purpose of use. Mycoplasma can be removed using a 0.1μm pore filter. Most culture media, buffer solutions, and routine laboratories typically use 0.22μm pore filters. Clarification and pre-filtration of solutions are generally performed with 0.45 μm filter membranes.
0.22μm filter membrane It is a microfiltration product, mainly made of filter elements made of various materials, and there are also laminated filter elements for deep filtration. At present, laminated filter elements are the main development trend. Laminated filter elements are also called membrane stacks. The outstanding advantage of this kind of membrane filtration for sterility inspection is that it can filter a larger amount of samples and filterable substances. The filtered test sample is It can be inoculated into the culture medium or observed directly with a microscope. Therefore, this method has high sensitivity, more reliable results than the “direct inoculation method”, is less likely to produce false negative results, and is relatively simple to operate. However, the entire process of sterility inspection should be strictly followed to prevent microbial contamination, so it is mostly performed in a laminar flow clean workbench.
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